產品概述
白細胞介素-6是在主體防御中起重要作用的多功能細胞因子，包括急性感染期、免疫應答、神經(jīng)細胞反應和造血作用。IL-6被常規(guī)細胞和轉化淋巴細胞、非淋巴細胞所表達。IL-6的表達上調被促有絲分裂信號、抗原刺激信號、脂多糖、鈣離子、細胞因子和病毒等眾多信號控制。IL-4、IL-10和IL-3調控IL-6在單核細胞中的表達。血清中IL-6水平已經(jīng)應用在大量的病理檢測中，這些檢測共包括細菌、病毒感染、外傷、免疫系統(tǒng)疾病和惡性腫瘤。大鼠IL-6 cDNA含211氨基酸殘基的多肽前體蛋白，其具有一個在成熟蛋白的第187氨基酸殘基位點的狂犬病信號肽。在蛋白序列水平上，大鼠IL-6和人的同源性約為39%，和小鼠IL-6的同源性為87%。
產品原理
本實驗采用雙抗體夾心ELISA法。抗大鼠IL-6單抗包被于酶標板上，同時加入標本、標準品和生物素化的抗大鼠IL-6抗體，標本、標準品中的IL-6會與加入于孔中的生物素化的抗大鼠IL-6抗體及包被于酶標板上的單抗結合，形成免疫復合物，游離的成分被洗去；加入辣根過氧化物酶標記的親合素，親合素與生物素特異性結合，游離的成分被洗去。加入顯色底物（顯色劑），若反應孔中有IL-6，辣根過氧化物酶會使無色的顯色劑變藍，加終止液變黃。在450nm處測OD值， IL-6濃度與OD450值之間呈正相關，可通過繪制標準曲線求出標本中IL-6濃度。
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