產(chǎn)品概述
胰島素是由胰島β細胞受內(nèi)源性或外源性物質(zhì)如葡萄糖、乳糖、核糖、精氨酸、胰高血糖素等的刺激而分泌的一種蛋白質(zhì)激素。由A、B鏈組成，共含51個氨基酸殘基。能增強細胞對葡萄糖的攝取利用，對蛋白質(zhì)及脂質(zhì)代謝有促進合成的作用。胰島素是機體內(nèi)唯一降低血糖的激素，也是唯一同時促進糖原、脂肪、蛋白質(zhì)合成的激素。
產(chǎn)品原理
本實驗采用雙抗體夾心ELISA法?？勾笫驣nsulin單抗包被于酶標板上，同時加入標本、標準品和生物素化的抗大鼠Insulin抗體，標本、標準品中的Insulin會與加入于孔中的生物素化的抗大鼠Insulin抗體及包被于酶標板上的單抗結合，形成免疫復合物，游離的成分被洗去；加入辣根過氧化物酶標記的親合素，親合素與生物素特異性結合，游離的成分被洗去。加入顯色底物（顯色劑），若反應孔中有Insulin，辣根過氧化物酶會使無色的顯色劑變藍，加終止液變黃。在450nm處測OD值，Insulin濃度與OD450值之間呈正相關，可通過繪制標準曲線求出標本中Insulin濃度。
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