產(chǎn)品概述

單核細胞趨化蛋白1(MCP-1/CCL2)，也稱為單核細胞趨化與激活因子（MCAF），是趨化因子家族CC亞族一個成員。MCP-1是一種很有潛能的單核細胞及巨噬細胞的誘生劑及激活因子。人MCP-1 cDNA編碼99個氨基酸組成的前體蛋白，當剪切掉23個氨基酸疏水性信號肽后，就形成了由76個氨基酸組成的成熟蛋白。與小鼠趨化因子相比，人MCP-1與小鼠MCP-5（CCL12）、MCP-1/JE(CCL2)最相關，小鼠MCP-5與人MCP-1有65%序列同源性。MCP-1可以被許多炎癥介導物誘導，如LPS、 IL-1β、 TNF-α、IFN-γ、IL-6及IL-4等。表達MCP-1的細胞有：單核細胞、肥大細胞、T細胞、成骨細胞、成纖維細胞、內(nèi)皮細胞、表皮細胞、小膠質細胞、星形膠質細胞及平滑肌細胞等。

產(chǎn)品原理

本實驗采用雙抗體夾心ELISA法?？谷薓CP-1單抗包被于酶標板上，標本和標準品中的MCP-1會與單抗結合形成免疫復合物，游離的成分被洗去；加入生物素化的抗人MCP-1抗體，生物素化抗人MCP-1抗體與酶標板上結合的標準品或樣本中的MCP-1結合而形成免疫復合物，游離的成分被洗去；加入辣根過氧化物酶標記的親合素，親合素與生物素特異性結合，游離的成分被洗去。加入顯色底物（顯色劑），若反應孔中有MCP-1，辣根過氧化物酶會使無色的顯色劑變藍，加終止液變黃。在450nm處測OD值， MCP-1濃度與OD₄₅₀值之間呈正相關，可通過繪制標準曲線求出標本中MCP-1濃度。

產(chǎn)品性能

1. 靈敏度：最小可測人MCP-1達7pg/ml 。

2. 重復性：板內(nèi)、板間變異系數(shù)均<10%。

產(chǎn)品組分及儲存條件

試驗所需自備儀器和試劑:

1. 酶標儀(450nm檢測波長濾光片，570nm或630nm校正波長濾光片)。

2. 高精度加液器及一次性吸頭：0.5-10μl，2-20μl，20-200μl，200-1000μl。

3. 37℃恒溫箱、蒸餾水或去離子水、坐標紙。

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Q:ResiQuant? Recombinant Protein A Residue Detection Kit （CRP00-3011）

A:ResiQuant? Recombinant Protein A Residue Detection Kit（ELISA Method）is used to quantitatively detect the residual natural or recombinant Protein A content in biopharmaceutical samples. Protein A includes natural Protein A, recombinant Protein A, and alkali-resistant recombinant Protein A which structure is significantly different from natural Protein A, such as GE’s MabSelect SuReTM Protein A. This product can quantitatively detect shed natural Protein A and recombinant Protein A, if you need detect alkaline-resistant recombinant Protein A, please choose our CRP00-302* series and CRP00-303* series kits. Product Advantages 1.Wide detection range: work range is 13.7-10000 pg/mL; 2.Easy to operate: Treat samples in a boiling water bath or mental bath; 3.Save time: sample processing only takes 10 minutes, and detection experiment takes about 2.5 hours; 4.High Sensitivity: the detection limit is 7 pg/mL, and the lower limit of quantification is 13.7 pg/mL; 5.High Precision: Intra-batch deviations were all ≤20%; 6.High Accuracy: sample spike recovery rate is in the range of 80%-120%; 7.Good Repeatability: within in the quantitative limit range, the CV values of sample concentrations within and between plates are ≤20%;

Q:ResiQuant? Alkali Resistant Protein A Residue Detection Kit 2G （CRP00-3032）

A:ResiQuant? Alkali-resistant Protein A Residue Detection Kit 2G（ELISA Method）can accurately quantify alkali-resistant recombinant Protein A. This product is a highly sensitive universal alkali-resistant Protein A quantitative detection kit, without interference, the lower limit of quantification can reach 4.9 pg/mL. Protein A includes natural Protein A, recombinant Protein A, and alkali-resistant recombinant Protein A which structure is significantly different from natural Protein A, such as GE’s MabSelect SuReTM Protein A. This product can quantitatively detect alkali-resistant recombinant Protein A. If you have higher requirements for sensitivity, please choose our CRP00-303* series kits. If you need to detect shed natural Protein A or recombinant Protein A, please choose our CRP00-301* series kits.