產(chǎn)品概述
基質(zhì)金屬蛋白酶(MMPs)是鋅依賴性降解細胞外基質(zhì)蛋白的內(nèi)肽酶家族中的一員。它以酶原(pro-MMPs)形式被分泌,可以激活多種蛋白酶或是在有機汞存在的條件下發(fā)揮作用。MMP的活性調(diào)控在組織重建、炎癥、腫瘤生成和轉(zhuǎn)移中起重要作用。單核細胞、巨噬細胞、嗜中性粒細胞、角化細胞、成纖維細胞、成骨細胞、軟骨細胞、骨骼肌星狀細胞、血管內(nèi)皮細胞和各種腫瘤細胞都可以分泌酶原 MMP -9。TGF-β 1,IL-1β,TGF-α,PDGF-AB,TNF-α和IL-1α可以使酶原MMP-9表達上調(diào)。 MMP-9 與炎癥反應、組織構(gòu)建、傷口愈合、生長因子和細胞因子過程有關。它的表達與伴隨胰腺癌生成的結(jié)締組織、與人乳腺癌細胞轉(zhuǎn)移到淋巴結(jié)和骨巨噬細胞瘤入侵局部導管相關。 MMP-9 在齦溝液、齒齦炎、牙周炎患者的唾液可能表達比較高。
產(chǎn)品原理
本實驗采用雙抗體夾心ELISA法??谷薓MP-9單抗包被于酶標板上,標本和標準品中的MMP-9會與單抗結(jié)合形成免疫復合物,游離的成分被洗去;加入生物素化的抗人MMP-9抗體,生物素化抗人MMP-9抗體與酶標板上結(jié)合的標準品或樣本中的MMP-9結(jié)合而形成免疫復合物,游離的成分被洗去;加入辣根過氧化物酶標記的親合素,親合素與生物素特異性結(jié)合,游離的成分被洗去。加入顯色底物(顯色劑),若反應孔中有MMP-9,辣根過氧化物酶會使無色的顯色劑變藍,加終止液變黃。在450nm處測OD值, MMP-9濃度與OD450值之間呈正相關,可通過繪制標準曲線求出標本中MMP-9濃度。
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